Sun F F, Taylor B M
Prostaglandins. 1981 Feb;21(2):307-14. doi: 10.1016/0090-6980(81)90148-9.
The metabolism of prostacyclin (PGI2) in vivo was investigated in Cynomolgus monkey. Following intravenous infusion of 11-[3H]-PGI2 for three days, pooled urine was extracted with Amberlite XAD-2, then chromatographed and purified by Sephadex LH-20, and reverse phase column chromatography. Radioactive fractions were converted to appropriate derivatives for identification by gas chromatography mass spectrometry. Twelve metabolites were characterized, the major of which was 6-keto-PGF1 alpha, accounting for 13% of the urinary radioactivity. The metabolic pathways are similar to those observed earlier in the rat. The excretion of substantial amounts of unchanged 6-keto-PGF1 alpha indicated that the monkey was not able to metabolize PGI2 as avidly as the rat.
在食蟹猴体内研究了前列环素（PGI2）的代谢。连续三天静脉输注11-[3H]-PGI2后，用Amberlite XAD-2提取收集的尿液，然后通过Sephadex LH-20和反相柱色谱进行色谱分离和纯化。将放射性组分转化为合适的衍生物，通过气相色谱-质谱联用进行鉴定。鉴定出了12种代谢物，其中主要的是6-酮-PGF1α，占尿中放射性的13%。代谢途径与早期在大鼠中观察到的相似。大量未变化的6-酮-PGF1α的排泄表明，食蟹猴不像大鼠那样能迅速代谢PGI2。
