A simple method for fusing human lymphocytes with rodent cells in monolayer by polyethylene glycol.

Human lymphocytes were efficiently hybridized with three rodent cell lines growing attached to a substrate, by a procedure similar to the monolayer fusion technique. Lymphocytes in suspension were directly added to the monolayer and cocultured for a few hours. Treatment with polyethylene glycol (PEG) induced close contact between the two cell types, and extensive cell fusion occurred. The rate of hybridization differed markedly for the different cell lines used.