在不同细胞密度下，用聚乙二醇融合的中国仓鼠和小鼠成纤维细胞的杂交频率。
Hybridization frequencies of Chinese hamster and mouse fibroblasts fused with polyethylene glycol at different cell densities.
作者信息
Lanfranchi G, Marin G
出版信息
Cell Differ. 1981 Mar;10(2):87-91. doi: 10.1016/0045-6039(81)90016-6.
PMID:7226233
Abstract
We have determined the effect of parental cell concentration at the time of fusion with polyethylene glycol on the frequency of viable somatic hybrids and of polykaryocytes of different multiplicity, between Chinese hamster (line Wg3) and mouse (line 3TP) fibroblasts. Fusion appeared to be dictated by the random encounter of cells only at low cell concentrations. However, no evidence for species-specific restriction of fusion was obtained at any cell concentration.
摘要
我们已经确定了中国仓鼠（Wg3系）和小鼠（3TP系）成纤维细胞在与聚乙二醇融合时亲本细胞浓度对不同多倍体活体细胞杂种和多核细胞频率的影响。仅在低细胞浓度下，融合似乎仅由细胞的随机相遇决定。然而，在任何细胞浓度下均未获得物种特异性融合限制的证据。
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