庆大霉素检测方法的比较。

Comparison of gentamicin assays.

作者信息

Oeltgen P R, Hamann S R, Blouin R A

出版信息

Ther Drug Monit. 1980;2(4):423-5.

Abstract

The reliability of the newly developed enzyme multiplied immunoassay technique (EMIT) for gentamicin with a radioimmunoassay (RIA) technique that utilizes a double antibody technique was compared. A split sample comparison was performed on 86 serum gentamicin levels from 35 patients. The Gilford System 4 and the Abbott Auto Log Gamma Counter were employed for the EMIT and RIA assays, respectively. Comparison of the two methods revealed good correlation (r = 0.954), slope (0.952), and an intercept of 0.017 over the range of the standard curve (1--16 microgram/ml). Within-run and between-run precision were comparable for both systems. Both assays have found widespread application for routine therapeutic monitoring of gentamicin. However, the EMIT gentamicin assay offers significant advantages in speed and simplicity while maintaining comparable accuracy and precision to that of the established RIA procedures.

摘要

将新开发的庆大霉素酶放大免疫分析技术（EMIT）与采用双抗体技术的放射免疫分析（RIA）技术的可靠性进行了比较。对35例患者的86份血清庆大霉素水平进行了样本分割比较。分别使用吉尔福德系统4和雅培自动对数伽马计数器进行EMIT和RIA检测。两种方法的比较显示，在标准曲线范围（1--16微克/毫升）内，相关性良好（r = 0.954），斜率为0.952，截距为0.017。两种系统的批内和批间精密度相当。两种检测方法都已广泛应用于庆大霉素的常规治疗监测。然而，EMIT庆大霉素检测在速度和简便性方面具有显著优势，同时保持了与既定RIA程序相当的准确性和精密度。