Lerche K H, Kretzschmar G
Z Allg Mikrobiol. 1980;20(10):641-52. doi: 10.1002/jobm.3630201007.
The electrical properties of the outer layer of the cell wall of a hydrocarbon-grown yeast C. guilliermondii were studied in detail by cell electrophoresis. Some experiments were also made with the yeast S. cerevisiae. The mobilities of the yeasts were measured as a function of pH at pH 2-11.5 and constant ionic strength I = 0.02 mol/l. For identification of surface groups the pH-mobility curves are used to calculate pK-values. The results indicate the presence of amino and carboxyl groups on the surface of C. guilliermondii. These groups are probably a part of a glyco-protein with an isoelectric point at pH 3.3 located on the surface. Electrophoresis of droplets of mineral oil coated with adsorbed protein layer confirms these conclusions. The importance of this glycoprotein in hydrocarbon uptake is discussed.
通过细胞电泳详细研究了烃类生长的酵母季也蒙毕赤酵母细胞壁外层的电学性质。还对酿酒酵母进行了一些实验。在pH 2 - 11.5且离子强度I = 0.02 mol/l恒定的条件下，测量酵母的迁移率作为pH的函数。为了鉴定表面基团，利用pH - 迁移率曲线来计算pK值。结果表明季也蒙毕赤酵母表面存在氨基和羧基基团。这些基团可能是位于表面的一种等电点为pH 3.3的糖蛋白的一部分。涂有吸附蛋白层的矿物油滴的电泳证实了这些结论。讨论了这种糖蛋白在烃类摄取中的重要性。
