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五种不同方法对β-溶血性链球菌进行分组的比较研究(作者译)

[Comparative study of five different methods for grouping beta-haemolytic streptococci (author's transl)].

作者信息

Lögering H J

出版信息

Zentralbl Bakteriol A. 1981 Feb;248(4):437-45.

PMID:7013362
Abstract

161 strains of beta-haemolytic streptococci, that had been isolated from patients' material, and 7 reference strains were group]ed with the techniques of precipitation, direct immunofluorescence, co-agglutination (Phadebact Streptococcus Test), latex agglutination (Streptex), and biochemical tests (Api-Strep). From the reference strains one group G strain was incorrectly identified with the Api-Strep. The direct immunofluorescence test yielded a result in agreement with the precipitation method in 94% of the freshly isolated strains. Co-agglutination and latex agglutination gave corresponding results with the precipitation method in 99%, taking into consideration that the first method doesn't apply to groups D and F. The biochemical determination with Api-Strep gave correct results with all strains of groups A and B, but did not do so with some group G and most of the group C streptococcal strains. Therefore especially co-agglutination and latex agglutination can be recommended for routine diagnostic purposes as reliable, rapid, and simple methods.

摘要

从患者样本中分离出的161株β溶血性链球菌以及7株参考菌株,采用沉淀法、直接免疫荧光法、协同凝集法(Phadebact链球菌试验)、乳胶凝集法(Streptex)和生化试验(Api-Strep)进行分组。在参考菌株中,有1株G群菌株通过Api-Strep鉴定错误。直接免疫荧光试验结果与沉淀法在94%的新鲜分离菌株中一致。考虑到协同凝集法不适用于D群和F群,协同凝集法和乳胶凝集法与沉淀法的对应结果为99%。Api-Strep生化测定对所有A群和B群菌株给出了正确结果,但对一些G群和大多数C群链球菌菌株则不然。因此,特别推荐协同凝集法和乳胶凝集法作为可靠、快速且简单的方法用于常规诊断目的。

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