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用于B、C和D组乳腺炎链球菌的商用乳胶凝集和协同凝集试剂的比较评估

Comparative evaluation of commercial latex agglutination and coagglutination reagents for groups B, C, and D mastitis streptococci.

作者信息

Poutrel B

出版信息

Am J Vet Res. 1983 Mar;44(3):490-2.

PMID:6404202
Abstract

A total of 144 strains of mastitis streptococci (groups B, C, and D) were grouped by conventional technique (Lancefield precipitin test) latex agglutination (LA), and coagglutination (CA). The LA correctly grouped 98% and the CA grouped 100% of strains on the test when the instructions of the manufacturers were followed. Sensitivity of both tests was improved for strains belonging to group D when extracts from colonies grown on sheep blood agar plates were used for grouping. Nonspecific reactions between groups C and B were observed only with one kit of LA reagents, whereas cross-reactions were recorded with all groups when CA reagents were used.

摘要

采用传统技术(兰斯菲尔德沉淀试验)、乳胶凝集试验(LA)和协同凝集试验(CA)对总共144株乳腺炎链球菌(B、C和D组)进行分组。按照制造商的说明进行操作时,LA对98%的菌株分组正确,CA对100%的菌株分组正确。当使用在绵羊血琼脂平板上生长的菌落提取物进行分组时,两种试验对D组菌株的敏感性均有所提高。仅在一套LA试剂中观察到C组和B组之间的非特异性反应,而使用CA试剂时,所有组之间均记录到交叉反应。

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引用本文的文献

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Streptococcus agalactiae mastitis: a review.无乳链球菌性乳腺炎:综述
Can Vet J. 1997 Jul;38(7):429-37.
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Identification of veterinary pathogens by use of commercial identification systems and new trends in antimicrobial susceptibility testing of veterinary pathogens.利用商业鉴定系统鉴定兽医病原体以及兽医病原体抗菌药敏试验的新趋势。
Clin Microbiol Rev. 1994 Jul;7(3):346-56. doi: 10.1128/CMR.7.3.346.
3
Evaluation of the API 20 Strep system for species identification of streptococci isolated from bovine mastitis.评价API 20 Strep系统对从牛乳腺炎分离出的链球菌进行菌种鉴定的能力。
J Clin Microbiol. 1984 Feb;19(2):213-4. doi: 10.1128/jcm.19.2.213-214.1984.
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Rapid differentiation of streptococci isolated from cows with mastitis.从患乳腺炎奶牛中分离出的链球菌的快速鉴别
J Clin Microbiol. 1986 Dec;24(6):1098-9. doi: 10.1128/jcm.24.6.1098-1099.1986.
5
Evaluation of the Rapid Mastitis Test for identification of Staphylococcus aureus and Streptococcus agalactiae isolated from bovine mammary glands.用于鉴定从牛乳腺分离出的金黄色葡萄球菌和无乳链球菌的快速乳腺炎检测方法的评估。
J Clin Microbiol. 1988 Apr;26(4):672-4. doi: 10.1128/jcm.26.4.672-674.1988.