Bologa-Sandru L, Siegrist H P, Z'Graggen A, Hofmann K, Wiesmann U, Dahl D, Herschkowitz N
Brain Res. 1981 Apr 6;210(1-2):217-29. doi: 10.1016/0006-8993(81)90895-7.
The expression of myelin basic protein (MBP) and galactocerebroside (GC), two antigenic markers for oligodendrocytes, was checked on 7-, 14-, 21- and 28-day-old dissociated mouse brain cell cultures (BCC) by using the indirect immunofluorescence method with double staining. The number of GC positive cells increased between the 7th and the 14th day of culture before a steady state was reached. In contrast to this, the MBP-positive cells appeared only on the 14th day of culture, and their number increased with the age of the culture. In double staining, the serum produced against isolated oligodendrocytes shows the same picture as the anti-GC serum, while only a part of GC-positive cells showed also the presence of MBP. Our data suggest that the GC appears very early on the membrane of the oligodendrocytes during development while cells exhibiting both GC and MBP probably represent a more differentiated oligodendrocyte population.
采用间接免疫荧光双染法，检测了7日龄、14日龄、21日龄和28日龄的解离小鼠脑细胞培养物（BCC）中少突胶质细胞的两种抗原标志物——髓鞘碱性蛋白（MBP）和半乳糖脑苷脂（GC）的表达情况。在达到稳定状态之前，培养第7天到第14天期间，GC阳性细胞数量增加。与此相反，MBP阳性细胞仅在培养第14天出现，且其数量随培养时间的延长而增加。在双染中，针对分离出的少突胶质细胞产生的血清呈现出与抗GC血清相同的结果，而只有一部分GC阳性细胞同时也表达MBP。我们的数据表明，在发育过程中，GC很早就出现在少突胶质细胞膜上，而同时表达GC和MBP的细胞可能代表了一个分化程度更高的少突胶质细胞群体。
