Jacque C M, Collet A, Raoul M, Monge M, Gumpel M
Brain Res. 1985 Aug;353(2):277-82. doi: 10.1016/0165-3806(85)90216-0.
The timing of myelin basic protein (MBP) expression and myelin component synthesis by the oligodendrocytes of the olfactory bulb was investigated in the mouse. Immunostaining with an anti-MBP immunoserum and a radioimmunoassay determination of MBP allowed to study the timing of MBP deposition during the development in this structure. Immunostaining of dissociated cells with anti-MBP and anti-galactosylceramide (anti-GC) was used to determine the state of development when these markers become expressed by olfactory bulb oligodendrocytes. Investigations using dissociated cells showed that GC-positive oligodendrocytes are already detected 3 days after birth in the olfactory bulb of the mouse and MBP is expressed 4 days later. Myelinated fibers were not visible on cryostat sections of olfactory bulb before 8 days postnatal. This work has been initiated by observations on the timing of myelination of olfactory bulb oligodendrocytes in transplantation experiments.
研究了小鼠嗅球少突胶质细胞髓鞘碱性蛋白（MBP）表达和髓鞘成分合成的时间。用抗MBP免疫血清进行免疫染色以及用放射免疫测定法测定MBP，有助于研究该结构发育过程中MBP沉积的时间。用抗MBP和抗半乳糖基神经酰胺（抗GC）对解离细胞进行免疫染色，以确定嗅球少突胶质细胞表达这些标志物时的发育状态。对解离细胞的研究表明，在小鼠出生后3天即可在嗅球中检测到GC阳性少突胶质细胞，4天后表达MBP。出生后8天之前，在嗅球的低温切片上看不到有髓纤维。这项工作是由移植实验中对嗅球少突胶质细胞髓鞘形成时间的观察引发的。
