Duchala C S, Asotra K, Macklin W B
Department of Psychiatry and Biobehavioral Science, UCLA Medical Center 90024, USA.
Dev Neurosci. 1995;17(2):70-80. doi: 10.1159/000111276.
Dissociated brain cell cultures are a useful model for investigating development and differentiation of oligodendrocytes in vitro. The current studies compare the developmental patterns of expression for oligodendrocyte lineage/myelin markers in both primary and secondary oligodendrocyte cultures derived from mouse and rat neonates. The rat and mouse dissociated brain cell cultures express the same myelin-specific antigens, but mouse oligodendrocytes produce a larger and more elaborate sheet-like membrane than rat oligodendrocytes, and some of the myelin markers (O4, GC, and MBP) show more intense membrane staining in mouse cultures. GD3 appears to be a good oligodendrocyte marker for rat cells, but it is nonspecific in mouse cells. There are fewer oligodendrocytes in mouse cultures, and they appear to require a longer differentiation time than rat oligodendrocytes. These same results are also observed in secondary oligodendrocyte cultures, although in general late myelin markers such as MBP and PLP are expressed at a much lower level in mouse cells than rat cells.
解离的脑细胞培养物是体外研究少突胶质细胞发育和分化的有用模型。当前研究比较了源自新生小鼠和大鼠的原代和二代少突胶质细胞培养物中少突胶质细胞谱系/髓鞘标志物的表达发育模式。大鼠和小鼠的解离脑细胞培养物表达相同的髓鞘特异性抗原，但小鼠少突胶质细胞产生的片状膜比大鼠少突胶质细胞更大且更精细，并且一些髓鞘标志物（O4、GC和MBP）在小鼠培养物中显示出更强的膜染色。GD3似乎是大鼠细胞的良好少突胶质细胞标志物，但在小鼠细胞中是非特异性的。小鼠培养物中的少突胶质细胞较少，并且它们似乎比大鼠少突胶质细胞需要更长的分化时间。在二代少突胶质细胞培养物中也观察到相同的结果，尽管一般来说，诸如MBP和PLP等晚期髓鞘标志物在小鼠细胞中的表达水平比大鼠细胞低得多。
