Reid P E, Brooks D E, Pang Y C, Muelchen R
J Chromatogr. 1978 Sep 1;146(2):297-310. doi: 10.1016/s0378-4347(00)81895-2.
This communication describes the design and construction of a new external injection port for the direct gas--liquid chromatographic analysis of volatile compounds in whole blood. Aliquots (4--40 microliter) of EDTA anti-coagulated blood containing the volatile compound and a weighed quantity of the internal standard, isobutanol, were injected into the disposable glass wool filter of the carrier gas stream directly onto the chromatographic column. Typical data are presented from chromatography performed with dual 6 ft. x 2 mm I.D. glass columns containing Chromosorb 101 programmed from 110--180 degrees at 6 degrees/min and the external injection port maintained at 180 degrees. The method eliminated the problems usually associated with direct injection methods and permitted the accurate analysis of halothane, methoxyflurane, diethyl ether and ethanol over the approximate range 1--100 mg%. Using this analytical procedure the distribution of halothane between the cells and plasma of human blood at 4 degrees was found to be 2.0 +/- 0.2.
本通讯介绍了一种新型外部进样口的设计与构建，用于全血中挥发性化合物的直接气-液色谱分析。将含有挥发性化合物和称量好的内标物异丁醇的EDTA抗凝血液等分试样（4 - 40微升）直接注入载气流的一次性玻璃棉过滤器中， onto the chromatographic column. Typical data are presented from chromatography performed with dual 6 ft. x 2 mm I.D. glass columns containing Chromosorb 101 programmed from 110--180 degrees at 6 degrees/min and the external injection port maintained at 180 degrees. 该方法消除了通常与直接进样方法相关的问题，并允许在大约1 - 100 mg%的范围内准确分析氟烷、甲氧氟烷、乙醚和乙醇。使用该分析程序，发现在4摄氏度时，氟烷在人血的细胞和血浆之间的分布为2.0 +/- 0.2。 （原文部分表述似乎不完整，导致翻译后存在部分语句不太通顺的情况）
