Morimoto K, Yoshikawa K, Yamaha T
Gan. 1980 Oct;71(5):674-8.
The alkyl-nitrosourea-mediated formation of interstrand cross-links in E. coli DNA was studied by means of hydroxyapatite column chromatography. DNA interstrand cross-links were detected after treatment with 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea (CCNU) but not after treatment with 1,1'-ethylene-bis(1-nitrosourea) (EBNU) or 1-methyl-1-nitrosourea (MNU). Thus, alkylation of DNA by EBNU seems to be monofunctional, as wtih MNU. The mutagenic and lethal activities of these three compounds were tested with a pair of Escherichia coli strains. EBNU gave slightly but significantly higher mutagenicity and lethality in a repairless (uvrA-) strain than in its parental strain and wild-type DNA repair, whereas CCNU and MNU showed equal mutagenicity and lethality in the two strains.
通过羟基磷灰石柱色谱法研究了烷基亚硝基脲介导的大肠杆菌DNA链间交联的形成。用1-(2-氯乙基)-3-环己基-1-亚硝基脲(CCNU)处理后可检测到DNA链间交联，但用1,1'-亚乙基双(1-亚硝基脲)(EBNU)或1-甲基-1-亚硝基脲(MNU)处理后未检测到。因此，与MNU一样，EBNU对DNA的烷基化似乎是单功能的。用一对大肠杆菌菌株测试了这三种化合物的诱变和致死活性。在无修复能力(uvrA-)的菌株中，EBNU的诱变率和致死率略高于其亲本菌株和野生型DNA修复菌株，而CCNU和MNU在这两种菌株中表现出相同的诱变率和致死率。
