Lung Wu H, Nakagawa T, Uno T
J Chromatogr. 1978 Sep 21;157:297-302. doi: 10.1016/s0021-9673(00)92346-x.
A gas chromatographic method has been established for the quantitative analysis of nalidixic acid. The method is based on the derivatization of nalidixic acid with diazomethane, and 5-alpha-cholestane is used as an internal standard. The sample is chromatographed on a glass column packed with 1% OV-1 on Chromosorb W. Quantitation is achieved by measuring peak-height ratios. The improved simplicity, specificity and accuracy of the method has been demonstrated for the quantitation of nalidixic acid in tablets.
已建立了一种气相色谱法用于萘啶酸的定量分析。该方法基于萘啶酸与重氮甲烷的衍生化反应，以5-α-胆甾烷作为内标。样品在填充有1% OV-1（涂覆于Chromosorb W上）的玻璃柱上进行色谱分析。通过测量峰高比实现定量。该方法在片剂中萘啶酸定量方面的改进后的简便性、特异性和准确性已得到证实。
