Strom J G, Jun H W
J Pharm Sci. 1977 Apr;66(4):589-90. doi: 10.1002/jps.2600660435.
A rapid and sensitive GLC method was developed for the quantitative determination of methenamine in tablets. The method was shown to possess several advantages over the official NF assay. After dissolution of the whole tablet in absolute ethanol and addition of an internal standard (pentylenetetrazol), an aliquot was injected into the gas chromatograph for analysis. The sample was chromatographed using a stainless steel column packed with 10% OV-17 on Chromosorb W-HP. Quantitation was achieved by measuring peak heights. The simplicity, directness, extreme rapidity, and accuracy of the method represents an improvement over the official method and the other proposed assays.
开发了一种快速灵敏的气相色谱法用于定量测定片剂中的乌洛托品。结果表明该方法相对于法定的美国国家处方集（NF）测定法具有多个优点。将整片药片溶解于无水乙醇中并加入内标物（戊四氮）后，取一份等分试样注入气相色谱仪进行分析。使用填充有10% OV - 17（固定液）的Chromosorb W - HP（担体）的不锈钢柱对样品进行色谱分析。通过测量峰高进行定量。该方法的简便性、直接性、极高的速度和准确性相较于法定方法及其他提出的测定法有了改进。
