Börzsönyi M, Surján A, Pintér A, Török G, Csik M, Tamás J, Fetter J, Ferencz A
IARC Sci Publ. 1980(31):677-83.
Trimorphamide [N-(1-formamido-2,2,2-trichloroethyl)morpholine], with an acute LD50 of 990 mg/kg, is a systemic fungicide under development. To examine its possible nitrosation in vivo, CFY rats were treated i.g. with 326 mg/kg trimorphamide, suspended in 40 mg/kg sodium nitrite. after 1 hour, another 40 mg/kg sodium nitrite was given i.g. and the animals were killed an hour later. The nitroso derivative was extracted by dichloromethane from the gastric content, after steam distillation. The samples were analysed by gas chromatography (GC) and mass spectrometry (MS). N-Nitrosomorpholine (NMOR) was identified by comparison with authentic samples, both by GC and MS, using retention times and electron impact mass spectra. Quantification was carried out by GC-MS-specific ion monitoring, using the m/e 116 peak. The yield of NMOR ws found to be 0.65%. By the use of the Ames test, NMOR formed in vivo proved to be strongly mutagenic for the strains TA 1535 and TA 100, both in presence and absence of S-9 fraction. Tumour-bearing animals, treated by sodium nitrite plus trimorphamide, are being analysed.
三甲吗啉[N-(1-甲酰胺基-2,2,2-三氯乙基)吗啉]，急性半数致死量为990毫克/千克，是一种正在研发的内吸性杀菌剂。为了检测其在体内可能的亚硝化作用，给CFY大鼠经口灌胃326毫克/千克三甲吗啉（悬浮于40毫克/千克亚硝酸钠中）。1小时后，再经口给予40毫克/千克亚硝酸钠，1小时后处死动物。经水蒸气蒸馏后，用二氯甲烷从胃内容物中提取亚硝基衍生物。样品通过气相色谱(GC)和质谱(MS)进行分析。通过与标准样品比较，利用保留时间和电子轰击质谱，通过GC和MS鉴定出N-亚硝基吗啉(NMOR)。使用m/e 116峰，通过GC-MS特定离子监测进行定量。发现NMOR的产率为0.65%。通过艾姆斯试验，发现在体内形成的NMOR无论在有还是没有S-9组分存在的情况下，对TA 1535和TA 100菌株都具有很强的致突变性。正在对用亚硝酸钠加三甲吗啉处理的荷瘤动物进行分析。
