甘油在某些口腔链球菌中的掺入。
Glycerol incorporation in certain oral streptococci.
作者信息
Kral T A, Daneo-Moore L
出版信息
Infect Immun. 1980 Dec;30(3):759-65. doi: 10.1128/iai.30.3.759-765.1980.
Abstract
Cells of 30 different strains of oral streptococci were grown in a chemically defined medium supplemented with [14C]glycerol to determine their ability to incorporate the labeled glycerol. Of the five species tested, only two, the rat-type strains (Streptococcus rattus) and strains isolated from wild rats (Streptococcus ferus), were able to incorporate the nonfermentable substrate, glycerol. For those strains capable of incorporating glycerol, the amount incorporated ranged from 0.15 to 0.43% of the cellular dry weight and followed simple saturation kinetics. The amount of glycerol incorporated depended solely on the concentration of glycerol in the growth medium. As a result, cultures exposed to low concentrations of glycerol ceased incorporation of the labeled glycerol before cessation of exponential growth.
摘要
在添加了[14C]甘油的化学限定培养基中培养30种不同口腔链球菌菌株的细胞，以确定它们摄取标记甘油的能力。在所测试的五个菌种中，只有两种，即大鼠型菌株（鼠链球菌）和从野生大鼠分离出的菌株（野鼠链球菌）能够摄取不可发酵底物甘油。对于那些能够摄取甘油的菌株，摄取量占细胞干重的0.15%至0.43%，并遵循简单的饱和动力学。摄取的甘油量仅取决于生长培养基中甘油的浓度。因此，暴露于低浓度甘油的培养物在指数生长停止之前就停止了对标记甘油的摄取。
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