将放射性大分子前体掺入粪链球菌的完整细胞和经渗透稳定处理的“原生质体”中。
Incorporation of radioactive macromolecular precursors into intact cells and osmotically stabilized "protoplasts" of Streptococcus faecalis.
作者信息
Roth G S, Daneo-Moore L
出版信息
J Bacteriol. 1971 Dec;108(3):980-5. doi: 10.1128/jb.108.3.980-985.1971.
Abstract
Growing "protoplasts" of Streptococcus faecalis were shown to incorporate newly administered radioactive precursors in the same manner as growing intact streptococci. No observable differences could be found between the size of the leucine precursor pools of the two cultures. The extent of turnover of protein and ribonucleic acid in both "protoplast" and streptococcal cultures appeared to be identical. Finally, the absolute rate of macromolecular biosynthesis was found to be equivalent whether determined on the basis of "new" or "old" label incorporation.
摘要
粪链球菌生长中的“原生质体”摄取新加入的放射性前体的方式，与生长中的完整链球菌相同。两种培养物的亮氨酸前体库大小没有明显差异。“原生质体”培养物和链球菌培养物中蛋白质和核糖核酸的周转程度似乎是相同的。最后，无论根据“新”标记还是“旧”标记掺入来测定，发现大分子生物合成的绝对速率都是相当的。
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