用于研究宿主因素与白色念珠菌相互作用的体内模型分析。
Analysis of an in vivo model to study the interaction of host factors with Candida albicans.
作者信息
Poor A H, Cutler J E
出版信息
Infect Immun. 1981 Mar;31(3):1104-9. doi: 10.1128/iai.31.3.1104-1109.1981.
Conditions were established under which membrane diffusion chambers surgically implanted into mice could be used to study interactions between host defense factors and Candida albicans within the chambers. Depending on the size of membrane pores, soluble host substances and phagocytic cells entered the chambers during the first 24 h after chamber implantation. By 7 days in vivo, the membranes of chambers appeared impermeable to these host factors. Mouse phagocytic cells were found to be functional within the in vivo chambers whether the cells emigrated to the chambers on their own accord or were placed there before chamber implantation. Opsonic factors such as antibody and complement appeared to be biologically functional within the in vivo chambers. Conditions suitable for harvesting C. albicans from the chambers also were determined.
建立了相应条件，在此条件下，通过外科手术植入小鼠体内的膜扩散小室可用于研究宿主防御因子与小室内白色念珠菌之间的相互作用。根据膜孔大小，可溶性宿主物质和吞噬细胞在小室植入后的最初24小时内进入小室。在体内7天时，小室的膜对这些宿主因子似乎变得不可渗透。发现小鼠吞噬细胞在体内小室内具有功能，无论细胞是自行迁移到小室还是在小室植入前就放置在那里。诸如抗体和补体等调理素因子在体内小室内似乎具有生物学功能。还确定了从小室中收获白色念珠菌的合适条件。
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