Lehrer R I, Cline M J
J Bacteriol. 1969 Jun;98(3):996-1004. doi: 10.1128/jb.98.3.996-1004.1969.
A quantitative assay of candidacidal activity based on differential staining of non-viable Candida albicans by methylene blue was developed and applied to studies of leukocytes from normal individuals and patients with fungal and other infections. Serum factors were necessary for optimal phagocytosis of C. albicans but lacked direct candidacidal activity. Normal human neutrophils (38 studies) killed 29.0 +/- 7.4% of ingested C. albicans in 1 hr. Eosinophils and monocytes killed a smaller percentage. Neutrophil candidacidal activity did not require protein or ribonucleic acid synthesis by the leukocyte but was inhibited by anaerobic conditions, potassium cyanide, and colchicine. Leukocytes of a patient with hereditary myeloperoxidase deficiency and of three children with chronic granulomatous disease phagocytized C. albicans normally, yet failed to kill them. Our data suggest that the neutrophil can play an important role in resistance to Candida infection and that the lysosomal enzyme myeloperoxidase and its oxidant substrate hydrogen peroxide are the major participants in neutrophil candidacidal activity.
基于亚甲蓝对非存活白色念珠菌的鉴别染色，开发了一种白色念珠菌杀菌活性的定量测定方法，并将其应用于对正常个体以及患有真菌和其他感染患者的白细胞研究。血清因子对于白色念珠菌的最佳吞噬作用是必需的，但缺乏直接的杀念珠菌活性。正常人类中性粒细胞（38项研究）在1小时内杀死了29.0±7.4%摄入的白色念珠菌。嗜酸性粒细胞和单核细胞杀死的比例较小。中性粒细胞的杀念珠菌活性不需要白细胞进行蛋白质或核糖核酸合成，但会受到厌氧条件、氰化钾和秋水仙碱的抑制。一名患有遗传性髓过氧化物酶缺乏症的患者以及三名患有慢性肉芽肿病的儿童的白细胞能够正常吞噬白色念珠菌，但无法将其杀死。我们的数据表明，中性粒细胞在抵抗念珠菌感染中可发挥重要作用，并且溶酶体酶髓过氧化物酶及其氧化底物过氧化氢是中性粒细胞杀念珠菌活性的主要参与者。
