Kotval J S, Christensen J R
J Virol. 1981 Mar;37(3):931-5. doi: 10.1128/JVI.37.3.931-935.1981.
At high multiplication of infection, a substantial fraction of restricting cells (P1 lysogens) could be productively infected by unmodified coliphage T1 (T1.0) provided that protein synthesis was uninhibited during the first 5 min of infection. Successful infection under restricting conditions was accompanied by more genetic recombination than was seen under nonrestricting host, the recombination frequency declined for markers on T1.0 genomes; no effect was seen on recombination between markers on modified (T1.P) genomes. This suggested that recombination between unmodified genomes may be essential for their survival under conditions of host restriction. In a restricting host, genetic markers on T1.0 could recombine with T1.P even when the rescuing phage was added 6 min after T1.0 infection. However, even marker rescue recombination was diminished when protein synthesis was inhibited during early infection. Since DNA restriction is an early event, protein synthesis may be required soon after infection of a restricting host by T1.0 in order to preserve restriction-damaged DNA in a form that can participate in recombination. Experiments are also described that rule out some possibilities for the role of such a protein(s).
在高感染复数时，如果在感染的最初5分钟内蛋白质合成未受抑制，相当一部分限制细胞（P1溶原菌）可能会被未修饰的大肠杆菌噬菌体T1（T1.0）有效感染。在限制条件下成功感染伴随着比在非限制宿主中更多的基因重组，T1.0基因组上标记的重组频率下降；对修饰（T1.P）基因组上标记之间的重组没有影响。这表明未修饰基因组之间的重组对于它们在宿主限制条件下的存活可能至关重要。在限制宿主中，即使在T1.0感染6分钟后添加拯救噬菌体，T1.0上的遗传标记也能与T1.P重组。然而，当早期感染期间蛋白质合成受到抑制时，即使是标记拯救重组也会减少。由于DNA限制是一个早期事件，T1.0感染限制宿主后可能很快就需要蛋白质合成，以便将受限制损伤的DNA保留在能够参与重组的形式中。还描述了一些实验，排除了这种蛋白质作用的一些可能性。
