限制性噬菌体T1的互补作用。
Complementation by restricted phage T1.
作者信息
Christensen J R
出版信息
J Virol. 1974 Dec;14(6):1411-8. doi: 10.1128/JVI.14.6.1411-1418.1974.
Abstract
The ability of restricted phage T1hr to complement stocks of T1am mutants carrying the P1 modification has been tested in mixed infection of Escherichia coli B(P1). Of the 18 genes tested, 15 could be complemented to give successful infection of approximately 10 to 25% of the cells, and 2 other genes consistently gave at least 5% complementation. The progeny phage produced in these infections was predominantly of the hr(+)am genotype. A mutant in gene 12 could be complemented very poorly, if at all. With one double mutant stock, the complementation obtained was nearly as good as the more poorly complemented of the two corresponding single mutants.
摘要
在大肠杆菌B(P1)的混合感染中，测试了限制性噬菌体T1hr对携带P1修饰的T1am突变体菌株的互补能力。在测试的18个基因中，15个基因可实现互补，从而成功感染约10%至25%的细胞，另外2个基因始终能实现至少5%的互补。这些感染中产生的子代噬菌体主要是hr(+)am基因型。基因12中的一个突变体几乎无法实现互补，即便能实现也程度极低。对于一种双突变体菌株，所获得的互补效果几乎与两个相应单突变体中互补效果较差的那个相当。
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