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[采用免疫酶技术(酶联免疫吸附测定)进行人布鲁氏菌病的血清学诊断。II.——与其他四种常用反应的比较(作者译)]

[Serological diagnosis of human brucellosis by means of an immunoenzymatic technic (ELISA). II.--Comparison with four other usual reactions (author's transl)].

作者信息

Marmonier A, Sthal J P, Micoud M, Le Noc P

出版信息

Pathol Biol (Paris). 1981 Feb;29(2):83-7.

PMID:7015241
Abstract

The authors analysing 553 serums, compare the results given by ELISA, in the diagnosis of human brucellosis, with four other usual serological tests : agglutination, rose Bengal plate agglutination test, complement fixation test, immunofluorescence. In the ELISA standard study and the research of specific M component, they can estimate, experimentally, the minimal absorbance necessary for concluding in positivity. They show the excellent specificity and sensitivity of this assay, its performances are almost the same as the immunofluorescence ones. Among 55 positive serums in one assay at least 83,6% are positive in both tests ELISA and IIF and 90,9% are detected by ELISA alone.

摘要

作者分析了553份血清,将酶联免疫吸附测定法(ELISA)在人类布鲁氏菌病诊断中给出的结果与其他四种常用血清学检测方法进行比较:凝集试验、玫瑰红平板凝集试验、补体结合试验、免疫荧光法。在ELISA标准研究和特定M成分的研究中,他们通过实验估计出判断为阳性所需的最小吸光度。他们展示了该检测方法出色的特异性和敏感性,其性能几乎与免疫荧光法相同。在至少一种检测方法中呈阳性的55份血清中,ELISA和间接免疫荧光法(IIF)检测均呈阳性的至少占83.6%,仅ELISA检测出阳性的占90.9%。

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