多头绒泡菌染色质5-S“单体”组分的特性分析。

The characterisation of a 5-S 'monosome' fraction from chromatin of Physarum polycephalum.

作者信息

Annesley M, Davies K E, Kumar N M, Walker I O

出版信息

Nucleic Acids Res. 1981 Feb 25;9(4):831-9. doi: 10.1093/nar/9.4.831.

DOI:10.1093/nar/9.4.831

PMID:7015286

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC326715/

Abstract

A so-called '5-S mononucleosome' fraction was isolated from nuclei of Physarum polycephalum by digestion with micrococcal nuclease and subsequent fractionation by gel filtration. This fraction had electrophoretic and sedimentation properties which were similar to DNA of approximately 140 base pairs in length. It is shown that lysis of the nuclei activates a protease which is resistant to phenyl-methyl sulphonyl fluoride, o-phenanthroline and parachloromercuri benzene sulphonate and which subsequently degrades the nucleosomes.

摘要

通过用微球菌核酸酶消化多头绒泡菌细胞核，随后进行凝胶过滤分级分离，从细胞核中分离出了一种所谓的“5-S单核小体”组分。该组分具有与长度约为140个碱基对的DNA相似的电泳和沉降特性。结果表明，细胞核的裂解激活了一种蛋白酶，该蛋白酶对苯甲基磺酰氟、邻菲啰啉和对氯汞苯磺酸盐具有抗性，随后会降解核小体。