临床分离的粘质沙雷氏菌的细菌素（marcescin）分型

Bacteriocin (marcescin) typing of clinically isolated Serratia marcescens.

作者信息

Nasu M

出版信息

Tohoku J Exp Med. 1981 Jan;133(1):33-43. doi: 10.1620/tjem.133.33.

DOI:10.1620/tjem.133.33

Abstract

A study of bacteriocin (marcescin) typing was carried out by an agar cross streaking method (without any induction reagent) with 654 strains of Serratia marcescens recently isolated from clinical materials in Nagasaki University Hospital. In a complete checker board experiment with 80 strains on bacteriocin production and sensitivity, 43 strains (54%) were productive, 74 (93%) were sensitive and 4 (5%) were negative. Immunity was confirmed in all strains. Eight out of 80 strains of Serratia marcescens were selected as indicators in order to achieve the best differentiation of strains in bacteriocin typing, and 654 strains were classified into 30 types by bacteriocin production typing and into 49 types by bacteriocin sensitivity typing; the former showed more stable results than the latter in reproducibility. Bacteriocins produced by this method were considered to be high molecular, phage tail-like group A bacteriocins reported by Prinsloo (1966). Bacteriocin production typing was more useful for classification and subdivision of strains than serotyping (0-group).

摘要

采用琼脂交叉划线法（不使用任何诱导试剂），对长崎大学医院近期从临床样本中分离出的654株粘质沙雷氏菌进行了细菌素（粘质菌素）分型研究。在对80株细菌素产生和敏感性的完整棋盘试验中，43株（54%）具有产生能力，74株（93%）敏感，4株（5%）呈阴性。所有菌株均证实有免疫性。为了在细菌素分型中实现菌株的最佳区分，从80株粘质沙雷氏菌中挑选出8株作为指示菌，654株菌株通过细菌素产生分型分为30型，通过细菌素敏感性分型分为49型；前者在可重复性方面比后者显示出更稳定的结果。用这种方法产生的细菌素被认为是Prinsloo（1966年）报道的高分子量、噬菌体尾样A组细菌素。细菌素产生分型在菌株的分类和细分方面比血清分型（O群）更有用。