Sproat D, Brown A
Am J Clin Pathol. 1979 Feb;71(2):172-6. doi: 10.1093/ajcp/71.2.172.
The authors describe a simplified system for the detection of bacteriocin production by Serratia marcescens with the use of six indicator strains, which include Escherichia coli, Klebsiella pneumoniae, Citrobacter diversus, Enterobacter aerogenes (two strains), and Serratia rubidaea grown on arabinose minimal medium plates. Of the 64 possible bacteriocin types, 11 were observed; 66% of the isolates tested were found to be one of three types. Occasionally more than one bacteriocin type was observed in an individual specimen; however, serotyping or antibiograms, or both, also indicated this was a different strain. The marcescin types were stable markers. With the use of this technic, different endemic strains of Serratia were shown to predominate in various areas of the hospital. In addition, when urinary tract isolates were compared with respiratory tract isolates, significant differences were found in the predominate types. The typing of these isolates by bacteriocin production was supported by serotype and antibiotype findings. The results suggest that this simple system may be a useful tool in a general hospital.
作者描述了一种简化系统，用于检测粘质沙雷氏菌产生的细菌素，该系统使用六种指示菌株，包括大肠杆菌、肺炎克雷伯菌、奇异柠檬酸杆菌、产气肠杆菌（两种菌株）以及在阿拉伯糖基本培养基平板上生长的深红沙雷氏菌。在64种可能的细菌素类型中，观察到了11种；测试的分离株中有66%被发现属于三种类型之一。偶尔在单个样本中会观察到不止一种细菌素类型；然而，血清分型或抗菌谱，或两者，也表明这是一个不同的菌株。粘质菌素类型是稳定的标志物。使用这种技术，不同的沙雷氏菌地方流行菌株在医院的各个区域占主导地位。此外，当将泌尿道分离株与呼吸道分离株进行比较时，在主要类型中发现了显著差异。通过血清型和抗菌型结果支持了通过细菌素产生对这些分离株进行分型。结果表明，这个简单的系统可能是综合医院中一个有用的工具。
