Alberte R S, Friedman A L, Gustafson D L, Rudnick M S, Lyman H
Biochim Biophys Acta. 1981 Apr 13;635(2):304-16. doi: 10.1016/0005-2728(81)90029-3.
The present study examined the protein associations and energy transfer characteristics of chlorophyll c and fucoxanthin which are the major light-harvesting pigments in the brown and diatomaceous algae. It was demonstrated that sodium dodecyl sulfate (SDS)-solubilized photosynthetic membranes of these species when subjected to SDS polyacrylamide gel electrophoresis yielded three spectrally distinct pigment-protein complexes. The slowest migrating zone was identical to complex I, the SDS-altered form of the P-700 chlorophyll a-protein. The zone of intermediate mobility contained chlorophyll c and chlorophyll a in a molar ratio of 2 : 1, possessed no fucoxanthin, and showed efficient energy transfer from chlorophyll c to chlorophyll a. The fastest migrating pigment-protein zone contained fucoxanthin and chlorophyll a, possessed no chlorophyll c, and showed efficient energy transfer from fucoxanthin to chlorophyll a. It is demonstrated that the chlorophyll a/c-protein and the chlorophyll a/fucoxanthin-protein complexes are common to the brown algae and diatoms examined, and likely share similar roles in the photosynthetic units of these species.
本研究检测了叶绿素c和岩藻黄质的蛋白质缔合及能量转移特性，这两种物质是褐藻和硅藻中主要的捕光色素。结果表明，这些物种经十二烷基硫酸钠（SDS）增溶的光合膜在进行SDS聚丙烯酰胺凝胶电泳时，产生了三种光谱特性不同的色素-蛋白质复合物。迁移最慢的区带与复合物I相同，即P-700叶绿素a-蛋白的SDS改变形式。迁移速度中等的区带中叶绿素c与叶绿素a的摩尔比为2:1，不含岩藻黄质，并显示出从叶绿素c到叶绿素a的高效能量转移。迁移最快的色素-蛋白质区带含有岩藻黄质和叶绿素a，不含叶绿素c，并显示出从岩藻黄质到叶绿素a的高效能量转移。结果表明，叶绿素a/c-蛋白复合物和叶绿素a/岩藻黄质-蛋白复合物在所检测的褐藻和硅藻中是常见的，并且可能在这些物种的光合单位中发挥相似作用。
