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在乙硫氨酸存在下生长的大肠杆菌合成的核糖体及核糖体RNA的特性。核糖体RNA在缺乏甲基化时的正常成熟。

Properties of ribosomes and ribosomal RNAs synthesized by Escherichia coli grown in the presence of ethionine. Normal maturation of ribosomal RNA in the absence of methylation.

作者信息

Chelbi-Alix M K, Expert-Bezançon A, Hayes F, Alix J H, Branlant C

出版信息

Eur J Biochem. 1981 Apr;115(3):627-34. doi: 10.1111/j.1432-1033.1981.tb06248.x.

DOI:10.1111/j.1432-1033.1981.tb06248.x
PMID:7016541
Abstract

Analysis of 16-S rRNA synthesized in Escherichia coli D10 (met-) incubated in a medium containing ethionine in place of methionine shows that it lacks most and probably all of the methyl groups present in normal 16-SrRNA but possesses the same 3'-OH, and 5'-phosphate terminal sequences as the latter. 23-S rRNA formed in ethionine-treated cells also contains normal terminal sequences. 5-S rRNAs of normal and ethionine-treated E. coli D10 are identical. These results lead to the conclusion that methylation of ribosomal precursor RNAs is not necessary for their maturation to products with normal chain lengths and does not influence the conformation of 16-S rRNA.

摘要

对在含有乙硫氨酸而非甲硫氨酸的培养基中培养的大肠杆菌D10（met-）合成的16 - S rRNA进行分析表明，它缺少正常16 - S rRNA中存在的大部分甚至可能是所有甲基基团，但具有与后者相同的3'-OH和5'-磷酸末端序列。在经乙硫氨酸处理的细胞中形成的23 - S rRNA也含有正常的末端序列。正常和经乙硫氨酸处理的大肠杆菌D10的5 - S rRNA是相同的。这些结果得出结论，核糖体前体RNA的甲基化对于它们成熟为具有正常链长的产物不是必需的，并且不影响16 - S rRNA的构象。

相似文献

1
Properties of ribosomes and ribosomal RNAs synthesized by Escherichia coli grown in the presence of ethionine. Normal maturation of ribosomal RNA in the absence of methylation.在乙硫氨酸存在下生长的大肠杆菌合成的核糖体及核糖体RNA的特性。核糖体RNA在缺乏甲基化时的正常成熟。
Eur J Biochem. 1981 Apr;115(3):627-34. doi: 10.1111/j.1432-1033.1981.tb06248.x.
2
Properties of ribosomes and RNA synthesized by Escherichia coli grown in the presence of ethionine. V. Methylation dependence on the assembly of E. coli 50 S ribosomal subunits.
J Mol Biol. 1979 Feb 5;127(4):375-95. doi: 10.1016/0022-2836(79)90228-6.
3
[Detection of N-epsilon, N-epsilon, N-epsilon-trimethyllysine in ribosomal protein L 11 by methylation of E. coli ribosomes synthesized in the presence of ethionine].[通过在乙硫氨酸存在下合成的大肠杆菌核糖体甲基化检测核糖体蛋白L11中的N-ε，N-ε，N-ε-三甲基赖氨酸]
C R Acad Hebd Seances Acad Sci D. 1974 Feb 11;278(7):951-3.
4
Properties of ribosomes and ribosomal RNA formed by a relaxed mutant of Escherichia coli during growth with ethionine.在乙硫氨酸存在下生长时，大肠杆菌的一个松弛突变体所形成的核糖体及核糖体RNA的特性。
J Mol Biol. 1968 Jan 28;31(2):191-207. doi: 10.1016/0022-2836(68)90439-7.
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Processing of the 17-S Escherichia coli precursor RNA in the 27-S pre-ribosomal particle.27-S前核糖体颗粒中17-S大肠杆菌前体RNA的加工
Eur J Biochem. 1976 Jan 15;61(2):433-42. doi: 10.1111/j.1432-1033.1976.tb10037.x.
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[Properties of ribosomes and RNA synthesized by Escherichia coli cultivated in the presence of ethionine. 1. Interactions between the ribosomal subunits 30 S and 50 S synthesized in the presence of ethionine].[在乙硫氨酸存在下培养的大肠杆菌合成的核糖体和RNA的性质。1. 在乙硫氨酸存在下合成的30S和50S核糖体亚基之间的相互作用]
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Properties of ribosomes and RNA synthesized by Escherichia coli grown in the presence of ethionine. 3. Methylated proteins in 50 S ribosomes of E. coli EA2.在乙硫氨酸存在下生长的大肠杆菌合成的核糖体和RNA的特性。3. 大肠杆菌EA2 50 S核糖体中的甲基化蛋白。
J Mol Biol. 1974 Jun 15;86(1):139-59. doi: 10.1016/s0022-2836(74)80013-6.
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Ribosomal cistrons in higher plant cells. I. A definitive scheme for the maturation pathway of the primary transcriptional product of ribosomal cistrons in Acer pseudoplatanus L. cells.
Biochim Biophys Acta. 1976 Jan 5;418(1):104-16. doi: 10.1016/0005-2787(76)90331-2.
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Altered protein hypothesis of mammalian ageing processes-II. Discrimination ratio of methionine vs ethionine in the synthesis of ribosomal protein and RNA of C57BL/6J mouse liver.哺乳动物衰老过程的蛋白质改变假说-II。C57BL/6J小鼠肝脏核糖体蛋白和RNA合成中蛋氨酸与乙硫氨酸的区分率。
Exp Gerontol. 1975 Apr;10(2):119-36. doi: 10.1016/0531-5565(75)90041-8.
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The effect of ethionine on ribonucleic acid synthesis in rat liver.乙硫氨酸对大鼠肝脏核糖核酸合成的影响。
Biochem J. 1975 Sep;150(3):335-44. doi: 10.1042/bj1500335.

引用本文的文献

1
snoRNPs: Functions in Ribosome Biogenesis.snoRNPs：核糖体生物发生中的功能。
Biomolecules. 2020 May 18;10(5):783. doi: 10.3390/biom10050783.
2
Molecular aspects of the in vivo and in vitro effects of ethionine, an analog of methionine.蛋氨酸类似物乙硫氨酸在体内和体外作用的分子层面研究
Microbiol Rev. 1982 Sep;46(3):281-95. doi: 10.1128/mr.46.3.281-295.1982.
3
In vitro methylation of Escherichia coli 16S ribosomal RNA and 30S ribosomes.大肠杆菌16S核糖体RNA和30S核糖体的体外甲基化
Proc Natl Acad Sci U S A. 1989 Jul;86(13):4902-6. doi: 10.1073/pnas.86.13.4902.
4
A paradigm for local conformational control of function in the ribosome: binding of ribosomal protein S19 to Escherichia coli 16S rRNA in the presence of S7 is required for methylation of m2G966 and blocks methylation of m5C967 by their respective methyltransferases.核糖体中功能局部构象控制的范例：在S7存在的情况下，核糖体蛋白S19与大肠杆菌16S rRNA的结合是m2G966甲基化所必需的，并通过各自的甲基转移酶阻止m5C967的甲基化。
Nucleic Acids Res. 1991 Dec;19(25):7089-95. doi: 10.1093/nar/19.25.7089.
5
rna12+, a gene of Saccharomyces cerevisiae involved in pre-rRNA maturation. Characterization of a temperature-sensitive mutant, cloning and sequencing of the gene.rna12 +，酿酒酵母中一个参与前体rRNA成熟的基因。温度敏感突变体的特性分析、该基因的克隆与测序。
Mol Gen Genet. 1992 Mar;232(2):304-12. doi: 10.1007/BF00280010.