de Groot C, Wormmeester J
Eur J Cell Biol. 1981 Apr;24(1):9-15.
The capping behaviour of surface immunoglobulin (sIg) on rabbit and mouse B lymphocytes was investigated in the direct immunofluorescence test. Rabbit B lymphocytes showed fewer caps than mouse lymphocytes. This was not due to a lower rate of cap formation, but to a smaller subpopulation of cells able to form caps. This subpopulation was age-dependent in rabbits; it increased from 25% of sIg positive cells at 2 to 3 months to 50% at 2 to 3 years of age. The capping rate was not significantly different in rabbits and C57B1 mice, but it was much higher in Swiss mice. Endocytosis did not occur at temperatures below 30 degrees C. In mouse B cells endocytosis occurred exclusively in the cap configuration. In rabbit B lymphocytes, on the other hand, two types of endocytosis were observed: first, a rather rapid endocytosis during the formation of the patch configuration, occurring on small lymphocytes, and second, a rather slow endocytosis during the cap configuration, occurring on large lymphocytes.
在直接免疫荧光试验中研究了兔和小鼠B淋巴细胞表面免疫球蛋白（sIg）的帽化行为。兔B淋巴细胞形成的帽比小鼠淋巴细胞少。这并非由于帽形成速率较低，而是由于能够形成帽的细胞亚群较小。在兔中，该亚群与年龄有关；它从2至3个月时sIg阳性细胞的25%增加到2至3岁时的50%。兔和C57B1小鼠的帽化速率无显著差异，但瑞士小鼠的帽化速率要高得多。在低于30摄氏度的温度下不会发生内吞作用。在小鼠B细胞中，内吞作用仅以帽的形式发生。另一方面，在兔B淋巴细胞中观察到两种内吞作用：第一，在小淋巴细胞上形成斑状结构时发生相当快速的内吞作用，第二，在大淋巴细胞上帽状结构形成时发生相当缓慢的内吞作用。
