肠道肽对新生大鼠胰岛细胞单层培养物葡萄糖刺激的胰岛素释放的影响。

Effect of gut peptides on glucose-stimulated insulin release by monolayer cultures of neonatal rat islet cells.

作者信息

Fujimoto W Y

出版信息

Horm Metab Res. 1981 Mar;13(3):135-8. doi: 10.1055/s-2007-1019199.

DOI:10.1055/s-2007-1019199

Abstract

Gastric inhibitory polypeptide (GIP), pancreatic polypeptide (PP), glucagon, vasoactive intestinal polypeptide (VIP), bombesin, neurotensin, substance P, and cholecystokinin octapeptide (CCK-OP) were examined for their effects upon glucose-stimulated insulin secretion in denervated and isolated islet cells, namely, monolayer cultures of dispersed neonatal rat pancreatic islet cells. Only glucagon (14 nM), GIP (10 and 20 nM), and CCK-OP (20 nM) enhanced glucose-stimulated insulin release during a 60-min incubation period. None of the others altered insulin secretion under the conditions employed, although reported to influence insulin release in other systems.

摘要

研究了胃抑制性多肽（GIP）、胰多肽（PP）、胰高血糖素、血管活性肠肽（VIP）、蛙皮素、神经降压素、P物质和八肽胆囊收缩素（CCK-OP）对去神经支配和分离的胰岛细胞（即分散的新生大鼠胰岛细胞单层培养物）中葡萄糖刺激的胰岛素分泌的影响。在60分钟的孵育期内，只有胰高血糖素（14 nM）、GIP（10和20 nM）和CCK-OP（20 nM）增强了葡萄糖刺激的胰岛素释放。在所采用的条件下，其他物质均未改变胰岛素分泌，尽管据报道它们在其他系统中会影响胰岛素释放。