Rowse-Eagle D, Watson H D, Tignor G H
J Clin Microbiol. 1981 May;13(5):996-7. doi: 10.1128/jcm.13.5.996-997.1981.
Formalin-fixed, Paraplast-embedded tissue sections mounted on subbed slides were digested with crude trypsin in modified Sorensen phosphate buffer containing sodium fluoride before immunofluorescence staining. This method prevented detachment of sections from slides during processing, thereby permitting immunofluorescence and histological examinations of adjacent serial sections.
将福尔马林固定、石蜡包埋在苏木精处理过的载玻片上的组织切片,在含有氟化钠的改良索伦森磷酸盐缓冲液中用粗胰蛋白酶消化后,再进行免疫荧光染色。该方法可防止切片在处理过程中从载玻片上脱落,从而能够对相邻连续切片进行免疫荧光和组织学检查。