Wilson M I, Crichton P B, Old D C
J Clin Pathol. 1981 Apr;34(4):424-8. doi: 10.1136/jcp.34.4.424.
Cultures of Escherichia coli of urinary origin were examined after storage for 12 to 15 years by a combination of the techniques of biotyping, resistotyping, haemagglutinin typing, and colicin typing (multiple typing) which allowed strain identification to be made even when variation in one or more typing characters had occurred in vivo. Biotyping and resistotyping used in conjunction were sufficient to allow identification of as many as 106 of 110 pairs of E. coli examined. In the four pairs of isolates for which it was necessary to extend strain identification profiles to include data from other typing methods, probable strain identification was achieved for all but one pair. The multiple typing approach seems useful for retrospective analysis of stored cultures of E. coli.
对源自尿液的大肠杆菌培养物进行了检查，这些培养物在储存12至15年后，采用了生物分型、抗药型分型、血凝素分型和大肠杆菌素分型（多重分型）技术相结合的方法。即使在体内一个或多个分型特征发生变异的情况下，这种方法也能实现菌株鉴定。联合使用生物分型和抗药型分型足以鉴定所检测的110对大肠杆菌中的106对。在四对分离株中，有必要扩展菌株鉴定谱以纳入其他分型方法的数据，除一对分离株外，其余三对均实现了可能的菌株鉴定。多重分型方法似乎对大肠杆菌储存培养物的回顾性分析有用。
