Crichton P B, Logan J M, Old D C
Department of Medical Microbiology, University of Dundee Medical School, Ninewells Hospital.
Epidemiol Infect. 1993 Aug;111(1):81-8. doi: 10.1017/s0950268800056703.
A two-tier miniaturized scheme of eight tests was devised for biotyping strains of Escherichia coli in microwell plates. Primary biotypes were defined by positive and negative reactions in tests for fermentation of raffinose, sorbose, dulcitol and 2-deoxy-D-ribose and for decarboxylation of ornithine when read after specified periods of incubation; subtypes were identified within primary biotypes according to results in secondary tests for rhamnose fermentation, lysine decarboxylation and motility. The method gave reproducible results on different occasions of testing. Among 100 E. coli strains from various sources, 26 of the 32 possible primary biotypes and 56 full biotypes, as defined by results in both primary and secondary tests, were identified, thus demonstrating a high index of strain discrimination (D = 0.98). The scheme is recommended as a simple, reliable, inexpensive and efficient method of differentiating strains of E. coli.
设计了一种用于在微孔板中对大肠杆菌菌株进行生物分型的包含八项测试的两级小型化方案。通过在特定培养时间后读取棉子糖、山梨糖、卫矛醇和2-脱氧-D-核糖发酵测试以及鸟氨酸脱羧测试中的阳性和阴性反应来定义主要生物型；根据鼠李糖发酵、赖氨酸脱羧和运动性的二级测试结果在主要生物型中鉴定亚型。该方法在不同测试场合下均能给出可重复的结果。在来自各种来源的100株大肠杆菌菌株中，鉴定出了32种可能的主要生物型中的26种以及由一级和二级测试结果定义的56种完整生物型，从而显示出较高的菌株鉴别指数（D = 0.98）。该方案被推荐为一种简单、可靠、廉价且高效的区分大肠杆菌菌株的方法。
