Larsson L I
J Histochem Cytochem. 1981 Mar;29(3):408-10. doi: 10.1177/29.3.7016977.
A novel, convenient immunocytochemical model system that allows simultaneous testing of multiple peptides and peptide fragments is presented. Screening of antisera for specificity and sensitivity and ready detection of multiple antibody subpopulations is possible with this system. Using the peroxidase-antiperoxidase method, a threshold sensitivity of 100 pg of peptide was attained with the best antisera available. Routine screenings of antisera employ amounts of peptides corresponding to 1.8--180 pmol--concentrations about a thousandfold lower than those used for absorption controls. Because of the minute amounts of antigens needed, specificity screening against rare or costly antigens now becomes feasible.
本文介绍了一种新颖、便捷的免疫细胞化学模型系统，该系统可同时检测多种肽和肽片段。利用此系统能够筛选抗血清的特异性和敏感性，并能快速检测多种抗体亚群。采用过氧化物酶-抗过氧化物酶方法，使用现有的最佳抗血清可达到100 pg肽的阈值灵敏度。抗血清的常规筛选所使用的肽量相当于1.8 - 180 pmol，其浓度比用于吸收对照的浓度低约一千倍。由于所需抗原量极少，针对稀有或昂贵抗原的特异性筛选现在变得可行。
