Hackenthal E, Taugner R
Histochemistry. 1983;77(2):201-7. doi: 10.1007/BF00506563.
Apparently monospecific antisera against the decapeptide angiotensin I (ANG-I) have been analyzed for their crossreactivity to the octapeptide angiotensin II (ANG-II). Whereas in the conventional displacement reaction of labeled ANG-I by ANG-II crossreactivities were in the order of 0.01%, the direct binding assay revealed crossreactivities of up to 20%. Both labeled ANG-I and ANG-II can be displaced completely from the antibody by an excess of either ANG-I or ANG-II. A similar relationship was seen with respect to the crossreactivity of ANG-II-antisera to ANG-I. - The data demonstrate that false positive immunocytochemical reactions may be obtained if the specificity of the reaction relies on the displacement reaction and the preabsorption of the antisera with the respective peptide. - These results may be pertinent for peptides other than the angiotensins.
显然，已经分析了针对十肽血管紧张素I（ANG-I）的单特异性抗血清与八肽血管紧张素II（ANG-II）的交叉反应性。在传统的用ANG-II置换标记的ANG-I的反应中，交叉反应性约为0.01%，而直接结合试验显示交叉反应性高达20%。过量的ANG-I或ANG-II都能将标记的ANG-I和ANG-II从抗体上完全置换下来。在ANG-II抗血清与ANG-I的交叉反应性方面也观察到了类似的关系。- 数据表明，如果反应的特异性依赖于置换反应以及用相应肽对抗血清进行预吸收，可能会得到假阳性免疫细胞化学反应。- 这些结果可能适用于血管紧张素以外的其他肽。
