Bird J W, Roisen F J, Yorke G, Lee J A, McElligott M A, Triemer D F, St John A
J Histochem Cytochem. 1981 Mar;29(3A Suppl):431-9. doi: 10.1177/29.3.431.
Primary cell cultures prepared from chick embryonic skeletal muscle and the rat myogenic line L6 were examined morphologically and biochemically during several stages of development. The L6 cells were cultured to provide three morphologically distinct populations: prefusion, postfusion, and a subclone of cells that did not fuse even at high density. Ultrastructural studies revealed the characteristic morphology of healthy myoblasts. Acridine orange staining and cytochemical localization of acid phosphatase suggest the presence of presumptive lysosomal material. Enzymatic studies of lysosomal cathepsins B, D, H, and L revealed unusually high enzyme specific activities in these homogeneous myoblast populations. No activity was detected for the two nonlysosomal enzymes Ca2+-proteinase and serine proteinase. It is suggested that the lysosomal apparatus and its complement of enzymes play a significant role in the differentiation of muscle myotubes.
对从鸡胚骨骼肌和大鼠成肌细胞系L6制备的原代细胞培养物在发育的几个阶段进行了形态学和生物化学检查。培养L6细胞以提供三个形态上不同的群体：融合前、融合后以及即使在高密度下也不融合的细胞亚克隆。超微结构研究揭示了健康成肌细胞的特征形态。吖啶橙染色和酸性磷酸酶的细胞化学定位表明存在推定的溶酶体物质。对溶酶体组织蛋白酶B、D、H和L的酶学研究表明，在这些同质的成肌细胞群体中酶的比活性异常高。未检测到两种非溶酶体酶钙蛋白酶和丝氨酸蛋白酶的活性。有人提出，溶酶体装置及其酶成分在肌肉肌管的分化中起重要作用。
