McComb D J, Ryan N, Horvath E, Kovacs K, Nagy E, Berczi I, Domokos I, Laszlo F A
J Natl Cancer Inst. 1981 Jun;66(6):1103-11. doi: 10.1093/jnci/66.6.1103.
With the use of electron microscopic morphology and immunochemistry, 5 tumors were studied: a spontaneous prolactin-producing adenoma (LEP rats); an estrogen-induced intrasellar tumor (R-Amsterdam rats); and 3 transplanted tumors, MtT.W10 and MtT.W5 (WF rats) and MtT.F4 (F344 rats). All tumors were derived from rat adenohypophysis and are known to secrete prolactin, growth hormone, or adrenocorticotropic hormone. The spontaneous tumor consisted of a uniform population of cells containing only immunoreactive prolactin. In the estrogen-induced tumor, prolactin and growth hormone were localized in separate cell types with the use of the immunoperoxidase technique. In the MtT.W10 tumor, both immunoreactive prolactin and growth hormone were observed in the same cell and in separate cell types. In the MtT.F4 and MtT.W5 tumors, one cell type was identified that was characterized by lack of morphologic differentiation, reduced secretory granule number, and inconclusive immunopositivity.
利用电子显微镜形态学和免疫化学方法，对5种肿瘤进行了研究：一种自发性分泌催乳素的腺瘤（LEP大鼠）；一种雌激素诱导的鞍内肿瘤（R - 阿姆斯特丹大鼠）；以及3种移植瘤，MtT.W10和MtT.W5（WF大鼠）和MtT.F4（F344大鼠）。所有肿瘤均源自大鼠腺垂体，并且已知会分泌催乳素、生长激素或促肾上腺皮质激素。自发性肿瘤由仅含有免疫反应性催乳素的单一细胞群体组成。在雌激素诱导的肿瘤中，利用免疫过氧化物酶技术发现催乳素和生长激素定位于不同的细胞类型中。在MtT.W10肿瘤中，在同一细胞和不同细胞类型中均观察到免疫反应性催乳素和生长激素。在MtT.F4和MtT.W5肿瘤中，鉴定出一种细胞类型，其特征为缺乏形态分化、分泌颗粒数量减少以及免疫阳性不确定。
