Ireland G W, Hawkins S E
Microbios. 1980;28(113-114):185-201.
During asexual reproduction in Volvox tertius, a single cell, the gonidium, contained within the parental spheroid, undergoes enlargement followed by multiple cleavages. A method has been developed for the isolation and maintenance of gonidia in vitro in paraffin chambers. In these chambers development of the gonidium to a multicellular spheroid and subsequent inversion was apparently normal. The paraffin chambers allowed the monitoring of the effects of inhibitors on development without the possible interference of the parental spheroid. Enlargement and division of isolated gonidia was prevented by actinomycin D, cycloheximide and streptomycin. Division but not enlargement was inhibited by 5-fluorouracil, mitomycin C and ethidium bromide. Colchicine at high concentrations prevented inversion, as did streptomycin, cycloheximide and actinomycin D.
在三生团藻进行无性繁殖时，亲代球体中包含的单个细胞，即生殖细胞，会先增大，随后进行多次分裂。现已开发出一种方法，可在石蜡培养室中体外分离和培养生殖细胞。在这些培养室中，生殖细胞发育成多细胞球体并随后翻转的过程显然是正常的。石蜡培养室能够监测抑制剂对发育的影响，而不会受到亲代球体可能的干扰。放线菌素D、环己酰亚胺和链霉素可阻止分离的生殖细胞增大和分裂。5-氟尿嘧啶、丝裂霉素C和溴化乙锭抑制分裂但不抑制增大。高浓度秋水仙碱会阻止翻转，链霉素、环己酰亚胺和放线菌素D也有同样的作用。
