异烟肼在酵母中诱导的有丝分裂基因转换。过渡金属和细胞生理条件的影响。

Mitotic gene conversion induced in yeast by isoniazid. influence of a transition metal and of the physiological conditions of the cells.

作者信息

Zetterberg G, Boström G

出版信息

Mutat Res. 1981 May;91(3):215-9. doi: 10.1016/0165-7992(81)90034-8.

Abstract

Isoniazid (INH) induced mitotic gene conversion at the trp5 locus of strain D7 of Saccharomyces cerevisiae. A suggested mechanism involving autoxidation of INH and production of hydrogen peroxide is supported by the facts that (i) the presence of a transition metal, Mn(II), greatly enhanced the effect of INH, (ii) cells stored for a long time in the refrigerator are much more sensitive to INH + Mn(II) than fresh cells probably due to loss of catalase and/or peroxidase activity, (iii) presence of S9 mix during the treatment eliminated the effect of INH + Mn(II).

摘要

异烟肼（INH）在酿酒酵母D7菌株的trp5位点诱导有丝分裂基因转换。一种涉及异烟肼自氧化和过氧化氢产生的推测机制得到了以下事实的支持：（i）过渡金属锰（II）的存在极大地增强了异烟肼的作用；（ii）长时间储存在冰箱中的细胞对异烟肼+锰（II）比新鲜细胞更敏感，这可能是由于过氧化氢酶和/或过氧化物酶活性丧失所致；（iii）处理过程中存在S9混合物消除了异烟肼+锰（II）的作用。