Chattopadhyay H, Chattopadhyay C, Natvig J B, Mellbye O J, Pahle J
Scand J Immunol. 1980;12(6):475-9. doi: 10.1111/j.1365-3083.1980.tb00093.x.
Spontaneous leucocyte migration-inhibitory factor (LIF) was assayed by an indirect system in which mononuclear cells (MNC) eluted from rheumatoid synovial tissues or isolated from peripheral blood of normal donors were cultured without antigen. The supernatants from these cultures and control supernatants, heated to 80 degrees C for 30 min to destroy the LIF activity, were used in the test. In seven out of eleven rheumatoid arthritis patients the eluted synovial MNC produced LIF spontaneously, whereas none of the twelve normal blood donors showed any such production. The ability to show spontaneous LIF production was primarily seen in the joint tissues from sero-negative patients.
通过间接系统检测自发性白细胞迁移抑制因子（LIF），该系统中，从类风湿性滑膜组织洗脱或从正常供体外周血分离的单核细胞（MNC）在无抗原的情况下进行培养。这些培养物的上清液以及加热至80摄氏度30分钟以破坏LIF活性的对照上清液用于测试。11例类风湿性关节炎患者中有7例，其洗脱的滑膜MNC自发产生LIF，而12例正常献血者均未表现出任何此类产生。自发产生LIF的能力主要见于血清阴性患者的关节组织。
