Hovdenes J, Lund H, Lea T, Egeland T
Institute of Immunology and Rheumatology, Rikshospitalet, Oslo, Norway.
Scand J Rheumatol. 1988;17(5):371-8. doi: 10.3109/03009748809105273.
Synovial fluid (SF) from rheumatoid arthritis patients (RA) and culture-supernatants of synovial tissue (ST) cells from RA patients and juvenile rheumatoid arthritis (JRA) patients were examined for their ability to promote B cell growth. SF was not suitable for studying B cell growth promotion because with the anti-mu driven assay system employed, all 15 samples strongly inhibited B cell proliferation. Supernatants of in vitro unstimulated ST cells from RA and JRA patients affected B cell growth in different ways, ranging from strong inhibition to moderate stimulation. Supernatants of unstimulated peripheral blood mononuclear cells (MNC) from healthy donors did not influence B cell proliferation. After phytohaemagglutinin (PHA) stimulation of the ST cells and normal MNC, culture supernatants of RA ST cells and normal MNC all stimulated B-cell growth, while culture supernatants of PHA-stimulated JRA ST cells displayed a variable picture. The differences between PHA-supernatants from RA, JRA and normal MNC were not statistically significant. These results indicate that the inflamed synovia of JRA and RA patients contain cells that can produce soluble factors with B cell growth promoting activity. Some of the data in the study suggest that these factors are produced in vivo and thus might be responsible for the observed B-cell activation in joints of these patients.
检测了类风湿性关节炎患者（RA）的滑液（SF）以及RA患者和青少年类风湿性关节炎（JRA）患者滑膜组织（ST）细胞的培养上清液促进B细胞生长的能力。由于采用抗μ驱动检测系统，所有15份SF样本均强烈抑制B细胞增殖，因此SF不适合用于研究B细胞生长促进作用。RA和JRA患者体外未刺激的ST细胞上清液对B细胞生长的影响各不相同，从强烈抑制到适度刺激。健康供体未刺激的外周血单个核细胞（MNC）上清液不影响B细胞增殖。在用植物血凝素（PHA）刺激ST细胞和正常MNC后，RA ST细胞和正常MNC的培养上清液均刺激B细胞生长，而PHA刺激的JRA ST细胞培养上清液表现不一。RA、JRA和正常MNC的PHA上清液之间的差异无统计学意义。这些结果表明，JRA和RA患者的炎症滑膜含有能够产生具有B细胞生长促进活性的可溶性因子的细胞。该研究中的一些数据表明，这些因子是在体内产生的，因此可能是这些患者关节中观察到的B细胞活化的原因。
