Rustagi P K, LoBuglio A F
J Immunol Methods. 1978;23(1-2):69-78. doi: 10.1016/0022-1759(78)90110-2.
This study characterizes a new phagocytic assay system utilizing technetium-99m sulfur colloid as the phagocytic particle. Uptake of sulfur colloid by human polymorphonuclear leukocytes is a time and temperature dependent process that requires glucose for optimal uptake. In contrast to many other systems, sulfur colloid phagocytosis appears to be serum and divalent cation independent. An attractive feature of this system is the 10-fold increase in particle uptake with phagocytosis as compared to that at zero time.
本研究描述了一种新的吞噬分析系统,该系统利用锝-99m硫胶体作为吞噬颗粒。人多形核白细胞对硫胶体的摄取是一个时间和温度依赖性过程,需要葡萄糖以实现最佳摄取。与许多其他系统不同,硫胶体吞噬作用似乎不依赖血清和二价阳离子。该系统的一个吸引人的特点是,与零时相比,吞噬作用时颗粒摄取增加了10倍。