Hanna R W, Lomas F E
Eur J Nucl Med. 1986;12(4):159-62. doi: 10.1007/BF00256913.
Human autologous leucocytes can be simply and reproducibly labelled by phagocytic engulfment of technetium 99m stannous colloid with high leucocyte-labelling efficiency (LLE), similar human biodistribution to indium-111-oxine labelled leucocytes and good cell viability. A mean particle size of 2.1 microns is optimal for phagocytosis and the most important parameter in maintaining reproducibly high neutrophil uptake. It is more critical than hitherto appreciated. When such variables as type of colloid, purity of starting materials, speed of mixer rotation when preparing colloid and labelling leucocytes, heparin concentration, freshness of colloid preparation, type of sterilising membrane filter used and incubation time of cells with colloid are rigidly controlled, consistent labelling efficiencies in excess of 90% can be obtained with neutrophil predominance. The lyophilised kit tested produced suboptimal results.
人类自体白细胞可以通过吞噬99m锝锡胶体进行简单且可重复的标记，白细胞标记效率（LLE）高，与铟-111-奥克辛标记的白细胞具有相似的人体生物分布，且细胞活力良好。平均粒径为2.1微米最适合吞噬作用，也是维持可重复的高中性粒细胞摄取的最重要参数。它比迄今所认识到的更为关键。当严格控制诸如胶体类型、起始材料纯度、制备胶体和标记白细胞时混合器的旋转速度、肝素浓度、胶体制剂的新鲜度、所用灭菌膜过滤器的类型以及细胞与胶体的孵育时间等变量时，中性粒细胞占优势的情况下可获得超过90%的一致标记效率。所测试的冻干试剂盒产生的结果并不理想。
