Zemlianukhina O A, Kolycheva V V, Nesmeianova M A
Biokhimiia. 1981 Jan;46(1):92-9.
Preincubation of cells in the presence of 4% ethanol accompanied by an increase of non-saturated cis-vaccenic acid content was shown to promote synthesis of alkaline phosphatase. Preincubation of cells in 0.1% hexanol reducing the level of this acid, on the contrary, leads to partial repression of the enzyme synthesis; the lag-phase of repression in the cells with a raised content of non-saturated cis-vaccenic acid and, consequently, with a greater fluidity of lipids was also shown to be reduced. Conversely, the reduction of lipid membrane fluidity on ethanol addition simultaneously with the repressing metabolite ortho-phosphate extends the lag-phase of repression and removes it partially during cell cultivation in the presence of ortho-phosphate. The impact of lipid composition variations on the synthesis and repression of alkaline phosphatase is discussed.
已表明，在4%乙醇存在下对细胞进行预孵育，伴随着不饱和顺式- vaccenic酸含量的增加，可促进碱性磷酸酶的合成。相反，在0.1%己醇中对细胞进行预孵育，降低了这种酸的水平，导致该酶合成的部分抑制；在不饱和顺式- vaccenic酸含量升高、因而脂质流动性更大的细胞中，抑制的延迟期也被证明缩短了。相反，在添加乙醇的同时添加抑制性代谢物正磷酸盐，导致脂质膜流动性降低，这延长了抑制的延迟期，并在正磷酸盐存在下的细胞培养过程中部分消除了该延迟期。讨论了脂质组成变化对碱性磷酸酶合成和抑制的影响。
