Severin A I, Nesmeianova M A
Mol Biol (Mosk). 1976 Nov-Dec;10(6):1369-77.
Protein content of membranes in wild type strains of E. coli K12 and K10 and in mutants defective in alkaline phosphatase regulator genes: E. coli C85 (R1-R2+p+) and E. coli C4(R1+R2-P+) under the conditions of repression and derepression of this enzyme was studied. Correlation between the content in membranes of minor component with the molecular weight 30,700 and the state of the regulatory system of alkaline phosphatase biosynthesis was shown. This protein was absent in the membranes of the repressed cells of wild type strains and in the membranes of nonrepressible (constitutive) mutant E. coli C4. Probably the protein with molecular weight 30,700 is a product of the regulatory gene R2 and its binding with the membrane determines its regulatory function.
研究了大肠杆菌K12和K10野生型菌株以及碱性磷酸酶调节基因缺陷型突变体（大肠杆菌C85（R1-R2+p+）和大肠杆菌C4（R1+R2-P+））在该酶的阻遏和去阻遏条件下膜的蛋白质含量。结果表明，分子量为30700的次要成分在膜中的含量与碱性磷酸酶生物合成调节系统的状态之间存在相关性。这种蛋白质在野生型菌株的阻遏细胞的膜中以及不可阻遏（组成型）突变体大肠杆菌C4的膜中不存在。分子量为30700的蛋白质可能是调节基因R2的产物，其与膜的结合决定了其调节功能。
