Oliff A, Ruscetti S, Douglass E C, Scolnick E
Blood. 1981 Aug;58(2):244-54.
The Friend virus complex contains a helper-independent retrovirus, F-MuLV, and a replication-defective retrovirus, SFFV. Murine erythroleukemia cell lines (MEL) have been isolated previously from the leukemic tissues of mice infected with such stocks of Friend virus complex. Since our laboratory has shown that either F-MuLV or SFFV can induce a lethal erythroproliferative disease, it has been unclear which of the properties of conventional MEL cells were causally related to the infection of these cells by F-MuLV and which properties were related to the infection of these cells by SFFV. In this article, we have focused on the helper-independent component of the Friend virus complex, F-MuLV clone 57, which has recently been purified by molecular cloning. We report a method for the reproducible isolation of a new type of murine erythroleukemia cell that can be isolate from mice infected with F-MuLV clone 57. We have designated these cells as 57 TP-1, indicating their origin from mice infected with F-MuLV clone 57 and their characteristics as transplantable erythroleukemia cells. Upon subcutaneous, intraperitoneal, or intravenous inoculation of these cells, 57 TP-1 cells grow in the hematopoietic organs of the recipient mice. No subcutaneous or intraperitoneal nodules occur. Both F-MuLV and a Friend MCF virus can be detected in these cells, but no evidence to SFFV can be found.
弗氏病毒复合体包含一种辅助病毒非依赖型逆转录病毒F-MuLV和一种复制缺陷型逆转录病毒SFFV。鼠红白血病细胞系（MEL）先前已从感染此类弗氏病毒复合体毒株的小鼠白血病组织中分离得到。由于我们实验室已表明F-MuLV或SFFV均可诱发致死性红细胞增殖性疾病，因此尚不清楚传统MEL细胞的哪些特性与F-MuLV感染这些细胞存在因果关系，哪些特性与SFFV感染这些细胞有关。在本文中，我们聚焦于弗氏病毒复合体的辅助病毒非依赖型成分F-MuLV克隆57，该克隆最近已通过分子克隆纯化得到。我们报告了一种可重复分离新型鼠红白血病细胞的方法，这种细胞可从感染F-MuLV克隆57的小鼠中分离得到。我们将这些细胞命名为57 TP-1，表明它们源自感染F-MuLV克隆57的小鼠，且具有可移植性红白血病细胞的特征。将这些细胞皮下、腹腔内或静脉注射后，57 TP-1细胞可在受体小鼠的造血器官中生长。不会出现皮下或腹腔内结节。在这些细胞中可检测到F-MuLV和一种弗氏MCF病毒，但未发现SFFV的证据。
