Troxler D H, Yuan E, Linemeyer D, Ruscetti S, Scolnick E M
J Exp Med. 1978 Sep 1;148(3):639-53. doi: 10.1084/jem.148.3.639.
Recent studies have indicated that both the replication-defective spleen focus-forming virus (SFFV) in the Friend virus complex and the helper-independent mink cell focus-inducing (MCF) viruses derived from AKR-murine leukemia virus (MuLV) are env gene recombinants between ecotropic virus and xenotropic virus. In an attempt to isolate additional env gene recombinants between Friend murine leukemia virus (F-MuLV) and xenotropic virus, we have inoculated cloned ecotropic F-MuLV into newborn NIH Swiss mice and analyzed MuLV released from preleukemic and leukemic spleens of infected mice. Two helper-independent MCF strains of F-MuLV have been isolated. Like the previously described AKR-MCF viruses, the Friend MCF viruses are env gene recombinants between an ecotropic virus (F-MuLV) and a mouse xenotropic virus, as shown by host range, interference pattern, and tryptic peptide analysis of the gp70s of these MuLV. Furthermore, RNA from the Friend MCF viruses hybridizes completely to cDNAsffv, a nucleic acid probe which detects that portion of SFFV which was not derived from P-MuLV. The ability to isolate replicating MCF viruses derived from F-MuLV FURTHER strengthens the parallels between the Friend erythroleukemia system and the AKR thymic leukemia system. Finally, the potential relationship of helper-independent env gene recombinants between F-MuLV and xenotropic virus to be highly leukemogenic SFFV is discussed.
最近的研究表明，Friend病毒复合物中复制缺陷型的脾集落形成病毒（SFFV）以及源自AKR - 鼠白血病病毒（MuLV）的无辅助病毒的貂细胞集落诱导（MCF）病毒都是嗜亲性病毒和异嗜性病毒之间的env基因重组体。为了分离Friend鼠白血病病毒（F - MuLV）和异嗜性病毒之间的其他env基因重组体，我们将克隆的嗜亲性F - MuLV接种到新生的NIH瑞士小鼠中，并分析了感染小鼠白血病前期和白血病脾脏释放的MuLV。已经分离出两种无辅助病毒的F - MuLV的MCF毒株。如同先前描述的AKR - MCF病毒一样，Friend MCF病毒是嗜亲性病毒（F - MuLV）和小鼠异嗜性病毒之间的env基因重组体，这通过这些MuLV的宿主范围、干扰模式以及gp70s的胰蛋白酶肽分析得以证明。此外，Friend MCF病毒的RNA与cDNAsffv完全杂交，cDNAsffv是一种核酸探针，可检测SFFV中并非源自P - MuLV的部分。从F - MuLV分离出可复制的MCF病毒的能力进一步加强了Friend红白血病系统和AKR胸腺白血病系统之间的相似性。最后，讨论了F - MuLV和异嗜性病毒之间无辅助病毒的env基因重组体与高致白血病性SFFV之间的潜在关系。
