St Charles M H, Frank D, Tanner C E
Immunology. 1981 Jul;43(3):441-5.
The non-pathogenic Trypanosoma lewisi depresses the ability of infected rat spleen cells to respond immunologically to sheep erythrocytes. A study was made to determine whether the parasite would produce this same phenomenon in vitro in Marbrook culture vessels with erythrocyte-primed spleen cells from normal rats; the antibody response of the spleen cells was measured by the plaque-forming cell assay. Soluble factors from 1 X 10(7) T. lewisi depress the ability of primed cells to form a secondary antibody response in vitro to sheep red cells. The intensity of the inhibitory activity depends on the stage of the life cycle of the parasite and can also be demonstrated using sonicated, frozen-thawed or boiled preparations of the organism. It is suggested that the immunodepressive substances may be a glycoprotein and that it (they) may arise both from the intracellular and from the extracellular compartments of the organism. It is likely that the host-parasite associations in trypanosomiasis are regulated by such soluble parasite factors.
非致病性的路氏锥虫会抑制受感染大鼠脾细胞对绵羊红细胞产生免疫反应的能力。开展了一项研究，以确定该寄生虫在体外的马尔布鲁克培养容器中，对来自正常大鼠的经红细胞致敏的脾细胞是否会产生同样的现象；通过空斑形成细胞试验来检测脾细胞的抗体反应。来自1×10⁷个路氏锥虫的可溶性因子会抑制致敏细胞在体外对绵羊红细胞形成二次抗体反应的能力。抑制活性的强度取决于寄生虫的生命周期阶段，使用该生物体的超声处理、冻融或煮沸制剂也能证明这一点。有人提出，免疫抑制物质可能是一种糖蛋白，并且它（它们）可能来自生物体的细胞内和细胞外区室。锥虫病中的宿主-寄生虫关联很可能受此类可溶性寄生虫因子的调节。
