Weislow O S, Wheelock E F
J Immunol. 1975 Jan;114(1 Pt 1):211-5.
The in vitro antibody plaque-forming cell (PFC) response of normal spleen cells exposed to sheep erythrocytes in Marbrook chambers was depressed by the addition of Friend virus (FV) leukemic spleen cells. Fewer than 10-5 leukemic cells inhibited the PFC response of 10-7 normal cells. This immunodepression could not be produced with sonicated, irradiated, or mitomycin C-treated leukemic cells or with cellfree FV. It could be blocked by FV immune serum but was unaffected by high titers of purified interferon. Interferon did not inhibit the immune response of normal spleen cells to sheep erythrocytes. Spleen cells from mice treated with statolon or chlorite-oxidized oxyamylose statolon but not Newcastle disease virus or poly rI:poly rC were resistant to immunodepression in vitro by FV leukemic spleen cells. The unique ability of statolon to prevent immunodepression by leukemic spleen cells may be the basis of its FV leukemo-suppressive activity in vivo.
在马尔布鲁克小室中，暴露于绵羊红细胞的正常脾细胞的体外抗体空斑形成细胞（PFC）反应，会因加入弗氏病毒（FV）白血病脾细胞而受到抑制。少于10^-5个白血病细胞就能抑制10^-7个正常细胞的PFC反应。这种免疫抑制不能通过超声处理、辐照或丝裂霉素C处理的白血病细胞或无细胞FV产生。它可被FV免疫血清阻断，但不受高滴度纯化干扰素的影响。干扰素不抑制正常脾细胞对绵羊红细胞的免疫反应。用链孢霉烯酮或亚氯酸盐氧化的羟淀粉链孢霉烯酮处理但未用新城疫病毒或聚肌苷酸:聚胞苷酸处理的小鼠的脾细胞，对FV白血病脾细胞的体外免疫抑制具有抗性。链孢霉烯酮预防白血病脾细胞免疫抑制的独特能力，可能是其在体内FV白血病抑制活性的基础。
