Sundqvist K G, Wanger L
Immunology. 1981 Jul;43(3):573-80.
Varying numbers of human blood lymphocytes were stimulated by concanavalin A (Con-A) in a constant medium volume. At 'low' cell densities DNA synthesis was proportional to the number of cells in the cultures whereas at 'high' densities DNA synthesis was considerably lower than expected. In the presence of non-mitogenic microcarrier beads, (diameter 195 micrometer) to which the cells attached, DNA synthesis was proportional or nearly proportional to the cell number in the cultures also at 'high' cell densities. The potentiating effect of beads on lymphocyte stimulation was particularly noteworthy in individuals showing a weak mitogen response. Another approach that yielded proportionality between DNA synthesis and cell number both at 'low' and 'high' cell densities was the use of culture vessels with a larger bottom area. Under such conditions the presence of beads did not augment DNA synthesis. These results suggest that the availability of non-cellular adhesive surface is a major limiting factor and cell density a major regulating factor in the control of lymphocyte activation. Anchorage of the cells to a surface may modulate the density dependent 'growth control mechanism' indirectly via an influence on cell-cell interaction. An alternative less-likely interpretation is that the contact with non-cellular surfaces directly gives rise to regulatory responses in lymphocytes or accessory cells.
在恒定的培养基体积中，用伴刀豆球蛋白A（Con - A）刺激不同数量的人血淋巴细胞。在“低”细胞密度下，DNA合成与培养物中的细胞数量成正比，而在“高”密度下，DNA合成比预期的要低得多。在存在细胞附着的非促有丝分裂微载体珠（直径195微米）的情况下，在“高”细胞密度下，DNA合成也与培养物中的细胞数量成正比或几乎成正比。在有丝分裂原反应较弱的个体中，珠对淋巴细胞刺激的增强作用尤为显著。另一种在“低”和“高”细胞密度下均能使DNA合成与细胞数量成正比的方法是使用底部面积更大的培养容器。在这种条件下，珠的存在不会增加DNA合成。这些结果表明，非细胞粘附表面的可用性是控制淋巴细胞活化的主要限制因素，而细胞密度是主要调节因素。细胞与表面的锚定可能通过影响细胞间相互作用间接调节密度依赖性“生长控制机制”。另一种可能性较小的解释是，与非细胞表面的接触直接引发淋巴细胞或辅助细胞中的调节反应。
