Sundqvist K G, Wanger L
Immunology. 1980 Dec;41(4):883-90.
Beads of polyacrylamide, latex or DEAE-Sephadex markedly augmented the stimulation of unfractionated or T-enriched lymphocytes by concanavalin A (Con A) or phytohaemagglutinin (PHA). The beads were not mitogenic in the absence of Con A or PHA. A prerequisite for the bead-induced augmentation was that the stimulated lymphocytes had been depleted of phagocytic and/or adherent accessory cells. The enhancing effect of beads was most pronounced during the initial 12 h after the beginning of lymphocyte stimulation, but not limited to this early phase of the growth period. The stimulation of lymphocytes in petri dishes of adhesive tissue culture plastic and non-adhesive bacterial plastic were compared. The magnitude of the stimulation on the non-adhesive surface was 10--50% lower than on the adhesive one, this difference being most pronounced at hyperoptimal mitogen concentrations. These results indicate that contact between some cell type and a solid surface can improve lymphocyte stimulation under experimental conditions when the number of phagocytic and adherent accessory cells is a limiting factor. The fact that cultivation on bacterial plastic, where adhesion and spreading were abolished, produced substantial stimulation (albeit reduced) demonstrates that substrate contact may be important, but is not a prerequisite, for lymphocyte activation.
聚丙烯酰胺、乳胶或二乙氨基乙基葡聚糖（DEAE - 葡聚糖）微珠显著增强了伴刀豆球蛋白A（Con A）或植物血凝素（PHA）对未分级淋巴细胞或富含T细胞的淋巴细胞的刺激作用。在没有Con A或PHA的情况下，这些微珠没有促有丝分裂活性。微珠诱导增强作用的一个前提条件是，受刺激的淋巴细胞已去除吞噬性和/或黏附性辅助细胞。微珠的增强作用在淋巴细胞刺激开始后的最初12小时最为明显，但并不局限于生长周期的这个早期阶段。比较了在粘性组织培养塑料培养皿和非粘性细菌塑料培养皿中对淋巴细胞的刺激情况。在非粘性表面上的刺激强度比在粘性表面上低10% - 50%，这种差异在促有丝分裂原浓度超最佳时最为明显。这些结果表明，当吞噬性和黏附性辅助细胞的数量是一个限制因素时，在实验条件下，某些细胞类型与固体表面之间的接触可以改善淋巴细胞的刺激作用。在细菌塑料上培养时，黏附和铺展被消除，但仍产生了显著的刺激作用（尽管有所降低），这一事实表明底物接触可能很重要，但不是淋巴细胞激活的先决条件。
